Muestra la distribución de disciplinas para esta publicación.
Publicaciones WoS (Ediciones: ISSHP, ISTP, AHCI, SSCI, SCI), Scopus, SciELO Chile.
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DOI | |||||||
Año | 2011 | ||||||
Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from peanut and prokaryotic expression was done on the gene, which provided a foundation for cultivating anti-A. flavus peanut varieties.
Ord. | Autor | Género | Institución - País |
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Shan, Shihua | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
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Zhang, Ting-ting | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
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Li, Chunjuan | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
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Yang, Chen | - |
Shandong Peanut Res Inst - China
NE Agr Univ - China Shandong Peanut Research Institute - China Northeast Agricultural University - China Shandong Academy of Agricultural Sciences - China |
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Yan, Caixia | Hombre |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
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Wan, Shu-bo | - |
Shandong Acad Agr Sci - China
Shandong Academy of Agricultural Sciences - China |
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1 | Shan, Shihua | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
2 | Zhang, Ting-ting | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
3 | Li, Chunjuan | - |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
5 | Yan, Caixia | Hombre |
Shandong Peanut Res Inst - China
Shandong Peanut Research Institute - China Shandong Academy of Agricultural Sciences - China |
6 | Wan, Shu-bo | - |
Shandong Acad Agr Sci - China
Shandong Academy of Agricultural Sciences - China |
Fuente |
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National Natural Science Foundation of China |
Modern Agro-industry Technology Research System |
National Key Project of Scientific and Technical Supporting Programs of China |