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DOI | 10.1128/AEM.00602-08 | ||||
Año | 2008 | ||||
Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and the organoleptic properties that they confer to wine. Here, we used a two-factor design to study the responses of a standard laboratory strain, CEN.PK113-7D, and an industrial wine yeast strain, EC1118, to growth temperatures of 15 degrees C and 30 degrees C in nitrogen-limited, anaerobic, steady-state chemostat cultures. Physiological characterization revealed that the growth temperature strongly impacted the biomass yield of both strains. Moreover, we found that the wine yeast was better adapted to mobilizing resources for biomass production and that the laboratory yeast exhibited higher fermentation rates. To elucidate mechanistic differences controlling the growth temperature response and underlying adaptive mechanisms between the strains, DNA microarrays and targeted metabolome analysis were used. We identified 1,007 temperature-dependent genes and 473 strain-dependent genes. The transcriptional response was used to identify highly correlated gene expression subnetworks within yeast metabolism. We showed that temperature differences most strongly affect nitrogen metabolism and the heat shock response. A lack of stress response element-mediated gene induction, coupled with reduced trehalose levels, indicated that there was a decreased general stress response at 15 degrees C compared to that at 30 degrees C. Differential responses among strains were centered on sugar uptake, nitrogen metabolism, and expression of genes related to organoleptic properties. Our study provides global insight into how growth temperature affects differential physiological and transcriptional responses in laboratory and wine strains of S. cerevisiae.
Ord. | Autor | Género | Institución - País |
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1 | Pizarro, Francisco J. | Hombre |
Pontificia Universidad Católica de Chile - Chile
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2 | Jewett, Michael C. | Hombre |
Tech Univ Denmark - Dinamarca
Danmarks Tekniske Universitet - Dinamarca Technical University of Denmark - Dinamarca |
3 | Nielsen, Jens | Hombre |
Tech Univ Denmark - Dinamarca
Danmarks Tekniske Universitet - Dinamarca Technical University of Denmark - Dinamarca |
4 | AGOSIN-TRUMPER, EDUARDO | Hombre |
Pontificia Universidad Católica de Chile - Chile
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Fuente |
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Otto Monsted |
Danish Research Agency for Production and Technology |
Center for Microbial Biotechnology, Biocentrum-DTU |
Chilean National Counsel for Scientific and Technologic Research |
DIPUC, Pontificia Universidad Catolica de Chile |
Agradecimiento |
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This research was supported by grants from the Danish Research Agency for Production and Technology and by the Chilean National Counsel for Scientific and Technologic Research (CONICYT project FONDECYT 1050688). M.C.J. was a recipient of an NSF International Research postdoctoral fellowship. A research stay for E.A. at DTU was funded by the Otto Monsted fund. A research stay for F.J.P. at DTU was funded in part by the Center for Microbial Biotechnology, Biocentrum-DTU, and by DIPUC, Pontificia Universidad Catolica de Chile. Kianoush K. Hansen, Joel Moxley, and Lene Christiansen are recognized for providing technical support and discussions. We thank John Villadsen for fruitful discussions. |