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DOI | 10.3389/FMICB.2018.01032 | ||||
Año | 2018 | ||||
Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a human fecal microbiota and (2) by successively transplanting aerotolerant anaerobic microorganisms, similar to the colonization in the human intestine during early life. We used conventionally raised zebrafish larvae harboring their own aerobic microbiota to improve the colonization of anaerobic microorganisms. The results showed with the fecal transplant, that some members of the human gut microbiota were transferred to larvae. Bacillus, Roseburia, Prevotella, Oscillospira, one unclassified genus of the family Ruminococcaceae and Enterobacteriaceae were detected in 3 days post fertilization (dpf) larvae; however only Bacillus persisted to 7 dpf. Successive inoculation of Lactobacillus. Bifidobacterium and Clostridioides did not improve their colonization, compared to individual inoculation of each bacterial species. Interestingly, the sporulating bacteria Bacillus clausii and Clostridioides difficile were the most persistent microorganisms. Their endospores persisted at least 5 days after inoculating 3 dpf larvae. However, when 5 dpf larvae were inoculated, the proportion of vegetative cells in larvae increased, revealing proliferation of the inoculated bacteria and better colonization of the host. In conclusion, these results suggest that it is feasible to colonize zebrafish larvae with some human bacteria, such as C. difficile and Bacillus and open an interesting area to study interactions between these microorganisms and the host.
Ord. | Autor | Género | Institución - País |
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1 | Valenzuela, Maria-Jose | Mujer |
Universidad de Chile - Chile
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2 | Caruffo, Mario | Hombre |
Universidad de Chile - Chile
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3 | Herrera, Yoani | - |
Universidad de Chile - Chile
UNIV MICHIGAN - Estados Unidos University of Michigan, Ann Arbor - Estados Unidos |
4 | Medina, Daniel A. | Hombre |
Pontificia Universidad Católica de Chile - Chile
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5 | Coronado, Maximo | - |
Universidad Nacional Andrés Bello - Chile
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6 | Feijoo, Carmen G. | Mujer |
Universidad Nacional Andrés Bello - Chile
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7 | Muñoz, Salomé | Mujer |
Universidad de Chile - Chile
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8 | GARRIDO-CORTES, DANIEL ANTONIO | Hombre |
Pontificia Universidad Católica de Chile - Chile
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9 | Troncoso, Miriam | Mujer |
Universidad de Chile - Chile
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10 | Figueroa, G | Hombre |
Universidad de Chile - Chile
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11 | Toro, Matteo | Hombre |
Universidad de Chile - Chile
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12 | Reyes-Jara, Angelica | Mujer |
Universidad de Chile - Chile
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13 | Magne, F. | Hombre |
Universidad de Chile - Chile
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14 | Navarrete, Paola | Mujer |
Universidad de Chile - Chile
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Fuente |
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CONICYT |
National Center of Minority Health & Health Disparities, National Institutes of Health |
INTA-University of Chile |
Dr. Stekel fellowship |
Agradecimiento |
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We would like to thank Dr. Lafayette Eaton for editing and revising the English grammar and Marcelo Balboa for his graphic support. The study was supported by grant ENLACE ENL010/15, grant from the INTA-University of Chile, and FONDECYT 3160525 (CONICYT). MarC acknowledges a Dr. Stekel fellowship, INTA-Nestle. We thank the Grant Number T37 MD001425-20 from the National Center of Minority Health & Health Disparities, National Institutes of Health and administered by the Center for Human Growth & Development of the University of Michigan. |