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DOI | 10.1038/SREP41798 | ||||
Año | 2017 | ||||
Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
The role of ions in the fungal decay process of lignocellulose biomaterials, and more broadly fungal metabolism, has implications for diverse research disciplines ranging from plant pathology and forest ecology, to carbon sequestration. Despite the importance of ions in fungal decay mechanisms, the spatial distribution and quantification of ions in lignocellulosic cell walls and fungal hyphae during decay is not known. Here we employ synchrotron-based X-ray fluorescence microscopy (XFM) to map and quantify physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn, in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood, fungal hyphae with the dried extracellular matrix (ECM) from the fungus, and Ca oxalate crystals. Three-dimensional ion volume reconstructions were also acquired of wood cell walls and hyphae with ECM. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cell wall length scales. These measurements provide new insights into the movement of ions during decay and illustrate how synchrotron-based XFM is uniquely suited study these ions.
Ord. | Autor | Género | Institución - País |
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1 | Kirker, Grant T. | Hombre |
US FOREST SERV - Estados Unidos
USDA Forest Products Laboratory - Estados Unidos |
2 | Zelinka, Sam | - |
US FOREST SERV - Estados Unidos
USDA Forest Products Laboratory - Estados Unidos |
3 | Gleber, Sophie-Charlotte | Mujer |
Argonne Natl Lab - Estados Unidos
The Advanced Photon Source - Estados Unidos |
4 | Vine, David | Hombre |
Argonne Natl Lab - Estados Unidos
The Advanced Photon Source - Estados Unidos |
5 | Finney, Lydia | Mujer |
Argonne Natl Lab - Estados Unidos
The Advanced Photon Source - Estados Unidos |
6 | Chen, Si | - |
Argonne Natl Lab - Estados Unidos
The Advanced Photon Source - Estados Unidos |
7 | Hong, Young Pyo | Mujer |
NORTHWESTERN UNIV - Estados Unidos
Northwestern University - Estados Unidos |
8 | Uyarte, Omar | Hombre |
UNIV SAO PAULO - Brasil
CMPC Celulosa - Chile Universidade de Sao Paulo - USP - Brasil Centro I+D - Chile Universidade de São Paulo - Brasil Centro I+D MetricArts - Chile |
9 | Vogt, Stefan | Hombre |
Argonne Natl Lab - Estados Unidos
The Advanced Photon Source - Estados Unidos |
10 | Jellison, Jody | Mujer |
Univ Massachusetts - Estados Unidos
University of Massachusetts Amherst - Estados Unidos |
11 | Goodell, Barry | Hombre |
Univ Massachusetts - Estados Unidos
University of Massachusetts Amherst - Estados Unidos |
12 | Jakes, Joseph E. | Hombre |
US FOREST SERV - Estados Unidos
USDA Forest Products Laboratory - Estados Unidos |
Fuente |
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U.S. Department of Energy |
Office of Science |
US Department of Energy |
Basic Energy Sciences |
USDA Hatch Multistate Project |
US Department of Energy, Basic Energy Sciences, Office of Science |
USDA PECASE awards |
FAPESP/BEPE Project |
Agradecimiento |
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The use of Advanced Photon Source facilities was supported by the US Department of Energy, Basic Energy Sciences, Office of Science, under contract number W-31-109-Eng-38. JEJ acknowledge funding from 2011 USDA PECASE awards. BG acknowledges support from USDA Hatch Multistate Project S-1041 VA-136288. OU acknowledge support from FAPESP/BEPE Project No 2013-04481-3. |
The use of Advanced Photon Source facilities was supported by the US Department of Energy, Basic Energy Sciences, Office of Science, under contract number W-31-109-Eng-38. JEJ acknowledge funding from 2011 USDA PECASE awards. BG acknowledges support from USDA Hatch Multistate Project S-1041 VA-136288. OU acknowledge support from FAPESP/BEPE Project N 2013-04481-3. |