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DOI | 10.1371/JOURNAL.PONE.0090626 | ||||
Año | 2014 | ||||
Tipo | artículo de investigación |
Citas Totales
Autores Afiliación Chile
Instituciones Chile
% Participación
Internacional
Autores
Afiliación Extranjera
Instituciones
Extranjeras
Unstable pathogenicity islands are chromosomal elements that can be transferred from one bacterium to another. Salmonella enterica serovar Enteritidis (S. Enteritidis) is a pathogenic bacterium containing such unstable pathogenicity islands. One of them, denominated ROD21, is 26.5 kb in size and capable of excising from the chromosome in certain culture conditions, as well as during bacterial infection of phagocytic cells. In this study we have evaluated whether ROD21 can be effectively transferred from one bacterium to another. We generated a donor and several recipient strains of S. Enteritidis to carry out transfer assays in liquid LB medium. These assays showed that ROD21 is effectively transferred from donor to recipient strains of S. Enteritidis and S. Typhimurium. When Escherichia coli was used as the recipient strain, ROD21 transfer failed to be observed. Subsequently, we showed that a conjugative process was required for the transfer of the island and that changes in temperature and pH increased the transfer frequency between Salmonella strains. Our data indicate that ROD21 is an unstable pathogenicity island that can be transferred by conjugation in a species-specific manner between Salmonellae. Further, ROD21 transfer frequency increases in response to environmental changes, such as pH and temperature.
Ord. | Autor | Género | Institución - País |
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1 | Salazar-Echegarai, Francisco J. | Hombre |
Pontificia Universidad Católica de Chile - Chile
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2 | Tobar, Hugo E. | Hombre |
Pontificia Universidad Católica de Chile - Chile
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3 | Nieto, Pamela A. | Mujer |
Pontificia Universidad Católica de Chile - Chile
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4 | RIEDEL-SORIA, CLAUDIA ANDREA | Mujer |
Universidad Nacional Andrés Bello - Chile
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5 | BUENO-RAMIREZ, SUSAN MARCELA | Mujer |
Pontificia Universidad Católica de Chile - Chile
INSERM - Francia Center for Research in Transplantation and Translational Immunology - Francia |
Agradecimiento |
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The authors are supported by the following grants: FONDECYT N. 11075060, FONDECYT N. 1110604, FONDECYT N. 1100971, FONDECYT N. 1110397, FONDECYT N. 1100926 and Millennium Institute on Immunology and Immunotherapy (P09-016-F). PAN is a CONICYT fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |